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. Author manuscript; available in PMC: 2018 Mar 5.
Published in final edited form as: J Investig Med. 2017 Nov 3;66(1):1–6. doi: 10.1136/jim-2017-000622

Figure 3.

Figure 3

Ubiquitin-specific protease 50 (USP50) is localized in the nuclei and its binding partners are identified. (A) Mouse lung epithelial (MLE12) cells were transfected with USP50-V5 plasmids and incubated for 24 hours. Cells were fixed and stained with a V5 antibody. USP50-V5: green; DAPI (4',6'-diamidino-2-phenylindole, nuclear DNA stain): blue. (B) HEK293T cells were transfected with USP50-V5 plasmid and incubated for 24 hours. Immunoprecipitation with a V5 antibody, followed by SDS-PAGE and LC-MS/MS analysis. Shown are potential USP50 binding partners separated by known intracellular location or function based on literature.