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. Author manuscript; available in PMC: 2018 Mar 5.
Published in final edited form as: J Investig Med. 2017 Nov 3;66(1):1–6. doi: 10.1136/jim-2017-000622

Figure 5.

Figure 5

Ubiquitin-specific protease 50 (USP50) is a not stable protein. (A) Mouse lung epithelial (MLE12) cells were transfected with USP50-V5 plasmids and incubated for 24 hours. Cells were treated with cycloheximide (CHX) (40 µg/mL) with or without MG132 (20 µg/mL) or leupeptin (20 µg/mL) for 0–2 hours. Cell lysates were analyzed by immunoblotting with antibodies against V5 and β-actin. (B) MLE12 cells were transfected with USP50-V5 and treated with 5 µg/mL lipopolysaccharide (LPS) for 5 hours. Total RNA was extracted and USP50 mRNA levels were determined by real-time qPCR. The expression of USP50 transcripts was normalized to Gapdh.