Figure 2.

Effect of LL-37 stimulation on TLR2 expression in mast cells. Mast cells were incubated with LL-37 at a final concentration of 1 μg/mL or medium alone (NS) for 1 h, 3 h, or 6 h. Representative images showing TLR2 cellular localization analyzed by confocal microscopy in (a, c) non- and (b, d) permeabilized cells. Single confocal sections (midsection of cells) reveal the surface and intracellular presence of TLR2. The signal was visualized with green Alexa 488. Fluorescence intensity diagrams showing the distribution of fluorescence in cells were mounted.