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. 2018 Mar 5;37:46. doi: 10.1186/s13046-018-0699-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Dysregulated of CADM2 mediated HCC cell migration and invasion. a-b Representative results of Wound-healing assays (P = 0.0008) and Transwell assays (for migration P = 0.0054, for invasion P = 0.0011) showed the effect of CADM2 expression on the migratory abilities and invasion of HCC cells. Column charts represent mean ± SEM of three independent experiments. Error bars represent SD. (*P < 0.05; **P < 0.01; ***P < 0.001) (c). Expression of EMT markers in HepG2 cells transfected with pEX-CADM2 or pEX-NC were examined by immunofluorescence. (Red: Vimentin, Green: E-cadherin, Blue: DAPI) (d). Western blotting analysis of indicated proteins in Hep3B cells transfected with pEX-CADM2 or pEX-NC. GAPDH was used as a loading control