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. 2018 Feb 19;2018:6853189. doi: 10.1155/2018/6853189

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Copyright © 2018 Paula A. Baldión et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

EXP-21 cells extracellular matrix (ECM) protein adhesion assay. Cell adhesion assay CytoSelect™ (Cell Biolabs) was employed to characterize cell attachment to ECM proteins like (b) fibronectin (FN), (c) type I-collagen (COL-I), (d) type IV-collagen (COL-IV), (e) laminin (LMN), (f) fibrinogen (FG). (a) Bovine serum albumin (BSA) is used as adhesion control. Attached cells after 1 h were stained with Coomassie blue dye which was subsequently extracted to compare the absorbances. The lowest adhesion was obtained on LMN. Data shown are mean ± SD of three independent experiments in duplicate (n = 6). Asterisks show significant differences (p < 0.05).