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. 2018 Mar 5;19:177. doi: 10.1186/s12864-018-4568-2

Fig. 12.

Fig. 12

The hepatic expression of 12 additional “candidate” genes was examined by qRT-PCR analysis in riboflavin-deficient and riboflavin-rescued embryos. The abundance of 12 additional genes was determined in an identical set of 32 RNA samples representing 4 biological samples/treatment (Rf + or Rf-) at 4 embryonic ages (e9, e11, e13 and e15). These gene expression levels are presented as arbitrary units (AU) and represent the mean ± SEM of four embryos/treatment and age. The Student’s t-test was used at each age to determine significant differences (P ≤ 0.05) between Rf + and Rf- embryos