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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Cell Microbiol. 2018 Jan 18;20(3):10.1111/cmi.12811. doi: 10.1111/cmi.12811

Fig 4. C. neoformans induces the phosphorylation of EphA2.

Fig 4

(A) Western blot analysis demonstrated the phosphorylation of EphA2 in brain endothelial cells when cells were challenged with C. neoformans (B) S. cerevisiae or (E) cps1Δ for 15min, 30min or 1h (middle panel). A polyclonal anti-phospho antibody to EphA2 was used to detect the phosphorylated form of EphA2. GAPDH was used as a loading control. (C) Endothelial cells treated with dasatinib and co-incubated with C. neoformans revealed a lack of EphA2 phosphorylation, however DMSO control clearly indicated phosphorylated EphA2, thus consistent with the notion that C. neoformans activates EphA2. (D, F) Relative band intensity of phosphorylated EphA2.