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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Cell Stem Cell. 2018 Mar 1;22(3):369–383.e8. doi: 10.1016/j.stem.2018.02.004

Figure 3. Inhibition of translation rapidly induces depletion of euchromatin marks in ESCs and blastocysts.

Figure 3

(A) Levels of indicated histone modifications upon 3 hours of CHX treatment at 0.1, 1 or 10μg/ml. See Figure S4A for biological replicates and quantifications. (B) Immunofluorescent detection and quantification of H4 acetylation (H4 K5/8/12) in DMSO- or CHX-treated ESCs. (C) Immunofluorescent detection of H4K16ac in control or CHX-treated (3 hours) E4.5 blastocysts and quantification in each Oct4+ cell (right panel). A representative z-section of each embryo is shown. (D) Correlation of CHX-induced H4K16ac changes with quartile of gene expression in ESCs (Bulut-Karslioglu et al., 2016). Profiles depict ChIP-seq tag density over annotated TSSs extended 2.5 kb upstream and downstream (3 hours CHX, 1 μg/ml). (E) Representative genome browser view depicting H4K16ac in DMSO- or CHX-treated cells over the ribosomal protein gene Rpl8. (F) ChIP-qPCR documenting a dose-dependent response of H4K16ac following 3 hours of CHX. Error bars show mean ± SD of 3 technical replicates. Scale bars denote 50 μm. Statistical tests are two-tailed t-tests with Welch’s correction. **, p<0.01; ****, p<0.0001.