Figure 1.

FOXA2 interacts with SIRT6 directly in vivo and in vitro.

Notes: (A) Total proteins from HepG2 cells were extracted, and coimmunoprecipitation experiments were performed, IP with antibodies against FOXA2 followed by IB with antibodies against SIRT6 or IP with SIRT6 followed by IB with FOXA2. (B) The relative co-immunoprecipitation experiments were performed in Huh7 cells. (C) GST pull-down assays were performed with the GST-fused FOXA2 protein and in vitro transcribed/translated SIRT6. (D) Total proteins from HepG2 cells were extracted, and coimmunoprecipitation experiments were performed, IP with antibodies against FOXA2 followed by IB with antibodies against SIRT1, SIRT5, or SIRT7.

Abbreviations: GST, glutathione-S-transferase; IB, immunoblotting; IP, immunoprecipitation; SIRT, sirtuin.