Figure 2.

FOXA2 and SIRT6 are downregulated in human HCC and cell lines.

Notes: (A) Total RNA was extracted from HCC tissues and adjacent noncancer tissues and subjected to RT-qPCR; the expression of FOXA2 was measured; n=20 in non-HCC group, and n=50 in HCC group. (B) mRNA level of SIRT6 was analyzed in the relative HCC tissues and adjacent noncancer tissues. (C) mRNA level of FOXA2 was analyzed in immortalized normal liver epithelial cells L02 and five HCC cell lines (HepG2, Huh7, Hep3B, SK-HEP-1, and PLC/PRF5). (D) mRNA level of SIRT6 was analyzed in the relative cell lines. All data were represented of three independent experiments. *P<0.05, **P<0.01.

Abbreviations: HCC, hepatocellular carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; SIRT, sirtuin.