Table 3.
Source of isolation and amino acid mutations of gyrA, parC, and mexR in correlation with fluoroquinolone susceptibility of clinical isolates of P. aeruginosa
| Type of specimen | No. of isolates | No. of isolates carrying mutations (fluoroquinolone susceptibility of both ciprofloxacin and levofloxacin)
|
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Susceptible
|
Intermediate
|
Resistant
|
|||||||||||||||||
|
gyrA
|
parC
|
mexR
|
gyrA
|
parC
|
mexR
|
gyrA
|
parC
|
mexR
|
|||||||||||
| 83 | 133 | 87 | 141 | 25 | 126 | 83 | 133 | 87 | 141 | 25 | 126 | 83 | 133 | 87 | 141 | 25 | 126 | ||
| Thr (ACC) | Thr (ACG) | Ser (TCG) | Ser (AGC) | Ile (ATC) | Glu (GAG) | Thr (ACC) | Thr (ACG) | Ser (TCG) | Ser (AGC) | Ile (ATC) | Glu (GAG) | Thr (ACC) | Thr (ACG) | Ser (TCG) | Ser (AGC) | Ile (ATC) | Glu (GAG) | ||
| Pus | 48 | — | — | — | — | — | Val (2) | — | — | — | — | — | Val (1) | Ile (28) | Met (13) | Leu (21) | His (1) Thr (1) |
Asn (4) Thr (2) |
Val (6) |
| Sputum | 59 | — | — | — | — | — | Val (7) | Ile (2) | Met (3) | — | — | — | — | Ile (26) | Met (23) | Leu (29) | — | Asn (2) Lys (1) |
Val (3) |
| Urine | 17 | — | — | — | — | — | Val (1) | — | — | — | — | — | — | Ile (11) | Met (6) | Leu (9) | — | — | Val (3) |
| Blood | 10 | — | — | — | — | — | — | Ile (1) | — | — | — | — | — | Ile (3) | Met (2) | Leu (5) | — | Leu (1) Pro (1) |
Val (1) |
| Peritoneal fluid | 3 | — | — | — | — | — | — | — | — | — | — | — | — | Ile (3) | Met (2) | Leu (3) | — | — | Val (1) |
| Bronchial lavage | 2 | — | — | — | — | — | — | — | — | — | — | — | — | — | Met (2) | — | — | — | Val (1) |
| Cerebrospinal fluid | 2 | — | — | — | — | — | — | — | — | — | — | — | — | Ile (2) | — | — | — | — | Val (1) |
| Total | 141 | 10 | 3 | 3 | 1 | 73 | 48 | 67 | 2 | 11 | 16 | ||||||||