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. Author manuscript; available in PMC: 2018 Mar 5.
Published in final edited form as: J Neuroimmune Pharmacol. 2011 Jun 14;6(4):585–596. doi: 10.1007/s11481-011-9284-5

FIGURE 10.

FIGURE 10

Acute ICV administration of IL-1β activates Fyn and PTK activities in splenic T cells. Rats (3/group) were implanted with ICV cannulas 1 wk prior to single injection of 5 μl of aCSF (CON) or IL-1β (50 ng/kg). Spleen cells were isolated and the extracts were resolved by Western blotting and visualized by anti-phosphotyrosine antibodies for PTK activity (A). The extracts were also immunoprecipitated with an anti-Fyn antibody, and the immunoprecipitates were used to determine Fyn kinase activity as described in Materials and Methods. Representative blots are shown in the figures.