Skip to main content
. 2017 Dec 18;80(2):272–279. doi: 10.1292/jvms.16-0408

Fig. 1.

Fig. 1.

Diagram of the chloroplast transformation vector. (A) pGEM-NNVOpt cloning vector harboring the codon-optimized coat protein gene; (B) Transformation vector TIA::RclpGAH. The vector consisted of two flanking regions (trnI and trnA) of which the sequences were the same as those of integration targets in the chloroplast genome and the coat protein gene driven by rice clp promoter. aadA: aminoglycoside 3′-adenylyltransferase; rrnB1/B2: terminator. Arrows indicating primers used to confirm the integration of coat protein gene and homoplastomy of transgenic plants.