Table 1. Immunization design, methods, and trial number in mouse.

Groups	Immunization methods	Formulae	Number
1–1	Feda)-only	90% normal diet+10% leaf powderb)	3
1–2		80% normal diet+20% leaf powder	3
2–1	Priming+fed	90% normal diet+10% leaf powder	3
2–2		80% normal diet+20% leaf powder	3
3–1	Priming+boosting+fed	90% normal diet+10% leaf powder	3
3–2		80% normal diet+20% leaf powder	3
4	Negative control	Normal diet	3

a) Oral immunization (fed) every Monday at 2-week intervals for a total of four times; b) Lyophilized transformant tobacco leaf powder (10% powder containing 1.5 µg of coat protein, while 3.0 µg for 20% powder).