Table 2. Immunization design, methods, and trial number in grouper.

Groups	Immunization methods	Formulae	Number
1	Negative control	Normal diet	15
2	Feda)-only	90% normal diet+10% leaf powderb)	15
3		80% normal diet+20% leaf powder	15
4	Priming+fed	90% normal diet+10% leaf powder	15
5		80% normal diet+20% leaf powder	15

a) Oral immunization (fed) every Monday at 2-week intervals for a total of 4 times; b) Lyophilized transformant tobacco leaf powder (10% powder containing 5 µg of coat protein, while 10 µg for 20% powder).