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. 2017 Dec 26;80(2):311–315. doi: 10.1292/jvms.17-0380

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©2018 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 2. — Protein expression comparison by Western blotting using anti-EHV-1 antibody (a). MDBK cells were infected with EHV-1 attB (lane 1), EHV-1∆VP22 (lane 2) and EHV-1∆VP22R (lane 3) at an MOI of 3 PFU/cell and analyzed by Western blotting with antibody to EHV-1 virions at 0, 2, 4 and 8 hrpi. Molecular sizes are shown on the left (a). Expression of ICP4s of EHV-1 attB, EHV-1∆VP22 and EHV-1∆VP22R were analyzed by Western blotting using anti-ICP4 antibody (b).