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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Oral Oncol. 2018 Feb 20;78:145–150. doi: 10.1016/j.oraloncology.2018.01.024

Fig. 4.

Fig. 4

Demonstration of in vitro killing of two head and neck cancer cell lines by CD70 CAR-T cells. (A) CD70-positive luciferase-expressing OQ01 (OQ01.Luc) and CAL27 (CAL27.Luc) cells were co-cultured with either CD70 CAR-T cells or non-transduced T cells (NT) at indicated effector-to-target (E:T) ratios for 24 hours. Specific killing activity of CD70 CAR-T cells was measured by bioluminescence assay. Cultures were set up in duplicate and mean ± error are shown. Similar results were obtained in independent experiments from three healthy T cell donors. (B) IFN-γ secretion of CD70 CAR-T cells in co-culture supernatants after 1 and 4 days was measured by ELISA. Cultures were set up in triplicate and mean ±SEM are shown. Statistical significance was determined by Student’s unpaired t test (*P<0.05; ***P<0.005).