Skip to main content
. 2018 Jan 22;19(3):188–197. doi: 10.1080/15384047.2017.1416274

Figure 1.

Figure 1.

Evaluation of OAd-mediated cytopathic effect (CPE) and TMZ induced-cytotoxicity in murine cancer cells. (A) Murine lung cancer KPCL and TC-1 cell lines were infected with Adhz60 at a multiplicity of infection (MOI) concentration of 0, 10, 25, 50 and 100. At 72h post-infection, crystal violet staining was used to evaluate CPE. A representative staining is shown of three experiments performed. (B) OAd-mediated CPE was calculated by measuring the absorbance of solubilized dye at 590 nm. Each point represents the mean of three independent experiments ± standard deviation (SD; bars). (C) Supernatants were collected and used to determine adenovirus yield from each cell line. Results represent the mean of three independent experiments ± standard deviation (SD; error bars) (*P < 0.05). (D) The cell lines above-mentioned were treated with TMZ at concentrations of 0, 50, 100, 200, and 400 µM. A MTT assay was used to determine cell survival at 72h post-treatment. Each point represents the mean of three independent experiments ± standard deviation (SD; bars).