Figure 2.

Effect of combined therapy of TMZ and OAd on virus replication in murine cancer and non-cancerous cells. (A) Murine cancer KPCL and TC-1 and non-cancerous MM14.Lu cells were treated with Adhz60 and TMZ at the following doses for Adhz60 and TMZ, respectively: (10 MOI, 400 µM). AdLacZ was used at 10 MOI for all cell lines. DMSO was added as a control at its respective volume for each cell line. At 72h post-infection, crystal violet staining was used to evaluate CPE. A representative staining is shown of three experiments performed. (B) OAd-mediated CPE was calculated by measuring the absorbance of solubilized dye at 590 nm. Results represent the mean of three repeated measurements ± standard deviation (SD; error bars) (*P < 0.05 for all cell lines). (C) Expression of adenovirus E1A proteins were detected with an anti-adenovirus type 5 E1A monoclonal antibody. Actin was used as a loading control; a representative experiment is shown from three performed. (D) Supernatants from Fig. 2A were used to determine adenovirus yield from each cell line. Results represent the mean of three independent experiments ± standard deviation (SD; error bars) (*P < 0.05).