Figure 3.

Evaluation of Adhz60 and TMZ combined therapy induced-apoptosis. Murine cancer cells were untreated or treated with DMSO, TMZ and Adhz60 alone or in combination as described in Fig. 2. (A) Seventy-two h post treatment, murine cancer cells were stained with annexin V-PE and 7-aminoactinomycin D (7-AAD). Positive cells for annexin V-PE and 7-AAD staining were analyzed by FACScan flow cytometer with FlowJo software. (B) Results represent the mean of three independent experiments ± standard deviation (SD; error bars) (*P < 0.05). (C) Whole cell protein lysates were collected 72h after indicated treatment. Expression of cleaved caspase-3 was detected by western blot; actin was used as a loading control. A representative experiment is shown from three performed.