Figure 6.

Effect of TMZ on Ad transduction efficiency and expression of coxsackie adenovirus receptor. Murine cancer cells were infected with AdGFP at an MOI of 10 followed by DMSO or TMZ at 400 µM. (A) Forty-eight h post treatment, cells were collected and GFP expression was analyzed by FACScan flow cytometer (Becton Dickinson, Franklin Lakes, NJ) and with FlowJo software (Tree Star Inc., Ashland, OR). A representative experiment is shown from three performed. (B) Expression of coxsackie adenovirus receptor was detected with an antibody that can recognize both human and mouse CAR. Actin was used as a loading control, a representative experiment is shown from three performed.