Table 2.

Summary of the bioassays that contributed to the study.

Laboratory code	Bioactivity	Target cell line	Source of Complement	Ratio E:Ta	Assay Type	Assay duration (h)b	In house reference standard	Assay read out (reagent)
1	CDCc	WIL2-S	Human	N/Ad	Viability	18	Yes	Fluorescence (Alamar Blue)
	ADCCe	WIL2-S	N/A	6	Reporter gene	6	Yes	Luminescence (Luciferase)
2	CDC	WIL2-S	Human	N/A	Viability	18	–	Fluorescence (Alamar Blue)
3	CDC	WIL2-S	Human	N/A	Viability	20 ± 4	Yes	Fluorescence (Alamar Blue)
4	CDC	WIL2-S	Human	N/A	Viability	18	–	Fluorescence (Alamar Blue)
5	CDC	WIL2-S	Rabbit	N/A	Viability	18 ± 2	Yes	Fluorescence (Alamar Blue)
6	CDC	Raji	Rabbit	N/A	Viability	2	Yes	Luminescence (Cell-titre Glo)
	ADCC	Raji	N/A	19	NK cell line Killing	1	Yes	Fluorescence (Calcein AM release)
	Binding	Raji	N/A	N/A	Competition binding	∼1	Yes	Fluorescence (FACs)
	Apoptosis	Z-138	N/A	N/A	Apoptosis	24 ± 1	Yes	Fluorescence (Annexin-V by FACS)
7	CDC	WIL2-S	Human	N/A	Viability	6	Yes	Colorimetric (MTS)
	ADCC	WIL2-S	N/A	7.6	Reporter gene	19	Yes	Luminescence (Luciferase)
8	CDC	Raji	Human	N/A	Viability	7	Yes	Colorimetric (CCK-8)
	ADCC	WIL2-S	N/A	5	Reporter gene	6	Yes	Luminescence (Luciferase)
	Binding	Raji	N/A	N/A	Binding	0.5	Yes	Fluorescence (2ry Ab detection by FACS)
9	CDC	Raji	Human	N/A	Toxicity	5	Yes	Colorimetric (LDH)
10	CDC	WIL2-S	Human	N/A	Viability	4–4.5	Yes	Colorimetric (CCK-8)
	ADCC	Raji	N/A	1	NK cell line Killing	2.5	Yes	Colorimetric (LDH)
	Binding	WIL2-S	N/A	N/A	Binding	1–1.5	Yes	Colorimetric (2ry Ab detection)
11	CDC	WIL2-S	Rabbit	N/A	Viability	2	Yes	Fluorescence (Alamar Blue)
	ADCC	WIL2-S	N/A	5:1	PBMC based Killing	4	–	Fluorescence (Cyto-tox reagent)
12	CDC	Daudi	Human	N/A	Toxicity	2	Yes	Luminescence (Cell-titre Glo)
	ADCC	Daudi	N/A	5	Reporter gene	4	Yes	Luminescence (Luciferase)
	Binding	Daudi	N/A	N/A	Binding	0.5	Yes	Fluorescence (2ry Ab detection by FACS)
13	CDC	WIL2-S	Rabbit	N/A	Toxicity	1	Yes	Fluorescence (Calcein AM release)
	ADCC	WIL2-S	N/A	25	NK cell line Killing	1	Yes	Fluorescence (Calcein AM release)
14	CDC	Jeko	Rabbit	N/A	Viability	2	–	Luminescence (Cell-titre Glo)
	ADCC	Jeko	N/A	8	Primary NK cell Killingb	18–22	–	Luminescence (Cyto-tox Glo)
15	CDC	WIL2-S	Human	N/A	Viability	2	Yes	Colorimetric (CCK-8)
	ADCC	Raji	N/A	16	PBMC based Killingb	4	Yes	Fluorescence (Calcein AM release)
16	CDC	WIL2-S	Human	N/A	Viability	18	Yes	Fluorescence (Alamar Blue)
	ADCC	WIL2-S	N/A	8	Dual-reporter gene	18	–	Luminescence (Firefly & Renilla Luciferase)
	Binding	WIL2-S	N/A	N/A	Binding	1	Yes	Fluorescence (2ry Ab detection by FACS)

^aE:T- effector: target cell ratio;

^b(h) hours;

^cCDC: Complement-dependent cytotoxicity;

^dN/A: non-applicable;

^eADCC: Antibody-dependent cell-mediated cytotoxicity.