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Methanol tolerance of the P. pastoris TK150 transketolase overexpressor strain. P. pastoris strains GS115 or TK150 (TK) were cultivated in 250 mL shake flasks in 50 mL BMMY media supplemented with methanol (MeOH) to the percentage v/v indicated. Samples were removed at the indicated time points and OD600 measured. Error bars indicate standard deviation among three biological repeats of the experiment.
