Figure 3.

Transketolase activity of TK50 strain cultivated in shake flasks. P. pastoris strains GS115 or TK150 (TK) were cultivated in 0.5% v/v methanol BMMY media. At the indicated time points samples were removed and prepared for incubation with 50 mM β‐hydroxypyruvate and 50 mM glycolaldehyde either as whole (WC) or disrupted cells (Dis), as described in Methods. The concentration of l‐erythrulose produced was measured by HPLC and plotted as a percentage of the starting concentration of substrates (50 mM), as a level of conversion. Error bars indicate standard deviation among three biological repeats of the experiment.