Figure 5.

Bioreactor cultivation of TK150 strain to high cell density. Strain TK150 was grown in a bioreactor to high cell density using a protocol in which methanol feeding commenced at the point post‐inoculation indicated by the M1 bar. Methanol feed rate is stepped up at M2 and subsequently at M3. Wet cell weight (WCW) and dry cell weight (DCW) for each sample are indicated (see inset key). Samples taken at zero, 33 and 58 h after the start of induction (M1) were used to test biocatalytic performance. Data set depicted is representative of three biological repeats of the experiment.