Figure 5.

UDCA decreases pro‐apoptotic miR‐34a levels in vesicle‐free serum fractions but not in liver tissue of morbidly obese patients. qRT‐PCR analysis of miR‐34a in vesicle‐free and exosome‐bound serum fractions of UDCA treated patients comparing day 1 (before treatment) and day 21 (end of treatment). UDCA day 1: n = 14; UDCA day 21: n = 14. (B) qRT‐PCR analysis of miR‐34a forward strand and miR‐34a reverse strand (miR‐34a*) in liver tissue of UDCA treated and untreated patients. Control: n = 18; UDCA: n = 19. (C) Immunoblotting of acetylated p53, total p53 and SIRT1. Signal intensities were normalized to beta‐actin and ratio of acetylated vs total protein was calculated. Representative Western blots are shown. Control: n = 7; UDCA: n = 6. Mean values ± SD are expressed for all data