Skip to main content
. 2018 Feb 7;13(1):95–107. doi: 10.1080/15592294.2017.1414128

Figure 2.

Figure 2.

DME-induced DNA demethylation is dependent upon the base excision repair (BER) pathway. (A) Non transfected cells and stably transfected lines were transiently transfected with SssI-methylated (right) or unmethylated (left) CMV-GFP reporter plasmid. Fluorescence microscopy images were taken 48 h after transfection. (B) Flow cytometry measurements of GFP expression. Values are shown relative to those detected in cells transiently transfected with unmethylated plasmid. Data are the mean ± SE of five independent experiments. (C) Effect of BER inhibitors on GFP reactivation. Cells transiently transfected with methylated CMV-GFP plasmid were plated either in the absence or presence of BER inhibitor (ABT or CRT; 50 mM), and GFP expression was measured after 24 h by flow cytometry. Values are shown relative to those detected in cells transiently transfected with unmethylated plasmid. Data are the mean ± SE of three independent experiments. Asterisks indicate statistical significance as determined by Student's t-test (* P < 0.05).