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. Author manuscript; available in PMC: 2018 May 8.
Published in final edited form as: Sci Transl Med. 2017 Nov 8;9(415):eaal2774. doi: 10.1126/scitranslmed.aal2774

Fig. 3. A circadian rhythm in fibroblast wound healing response.

Fig. 3

A. Fibroblast monolayers derived from adult PER2::LUC mouse skin were entrained and wounded after 20-64 hours in free run (i). (ii) Images of wound healing assays; time at wounding is indicated, residual wound is indicated by pink highlighting (scale bar = 500 μm). (iii) Quantification of the residual wound after 16 hours of wound healing (line, mean±SEM, n=4) with 3 parallel PER2::LUC measurements (heat maps). RAIN p-value indicated. Bi. Fibroblasts labelled with CellTracker Red healing after wounding at the indicated time after synchronisation (t). Scale=100 μm. Wound healing (ii) and leading cell velocity (iii) are quantified (n=4 or 5, ±SEM). P values from Tukey’s multiple comparisons test after 60 hours of healing (tH60) (ii) or tH0 (iii) are indicated. Ci Fibroblasts labelled with SiR-actin (red) and CellTracker Green (cyan) treated as in B (scale bar = 50 μm). A single cell for each condition has been highlighted in white with time healing indicated. The centre of mass for each label (ii) was determined over 9 hours of healing and the mean (±SEM) degree of polarisation (Δx) is indicated (iii). The P value from a two-way analysis of variance (ANOVA) is indicated.