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. Author manuscript; available in PMC: 2018 Mar 5.
Published in final edited form as: Cell Metab. 2017 Nov 9;27(1):85–100.e8. doi: 10.1016/j.cmet.2017.10.006

Figure 4. Metabolite NAD+ Regulates Hybrid Th1/17 Cell Function.

Figure 4

Purified CD4+ T cells differentiated to Th1, Th17, and Th1/17 were used for quantifying intracellular metabolite levels using mass spectrometry. The principle component analysis (PCA) is shown in (A), and (B) shows relative levels of metabolite. Th1, Th17, and Th1/17 cells differentiated in presence of either vehicle control (DMSO) or FK866 (10 nM) were used for determining (C) intracellular cytokine secretion by FACS, (D) expression of stemness-associated genes, and (E) ability to control growth of tumor s.c. established B16-F10-HLA-A2+ murine melanoma cells upon adoptive transfer. Tumor growth was measured using digital calipers every fourth day. Data demonstrate mean tumor size at each time point in one of the two experiments, with similar results. *p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.0001. Also see Figure S4.