| Antibodies |
| Anti-mouse CD3 |
BioXCell |
Clone: 145-2C11; Cat# BE0001-1; RRID: AB_1107634 |
| Anti-mouse CD28 |
BioXCell |
Clone: 37.51; Cat# BE0015-1; RRID: AB_1107624 |
| Anti-mouse IL4 |
BioXCell |
Clone: 11B11; Cat# BE0045; RRID: AB_1107707 |
| Anti-mouse IFNγ |
BioXCell |
Clone: XMG1.2; Cat# BE0055; RRID: AB_1107694 |
| CD4-PE |
eBioscience |
Clone: GK5.1; Cat# 12-0041-83; RRID: AB_465506 |
| T-bet-Percp/Cy5.5 |
eBioscience |
Clone: 4B10; Cat# 45-5825-80; RRID: AB_953657 |
| RORγ-APC |
eBioscience |
Clone: AFKJS-9; Cat# 17-6988-80; RRID: AB_10609207 |
| IRF-4-eFluor 660 |
eBioscience |
Clone: 3E4; Cat# 50-9858-80; RRID: AB_2574393 |
| CXCR3-PE |
eBioscience |
Clone: CXCR3-173; Cat# 12-1831-80; RRID: AB_1210734 |
| CD45.2-PE |
eBioscience |
Clone: 104; Cat# 12-0454-81; RRID: AB_465678 |
| CD39-PE/Cy7 |
eBioscience |
Clone: eBioA1 (A1); Cat# 25-0391-80; RRID: AB_1582280 |
| CD90.1-APC |
eBioscience |
Clone: HIS51; Cat# 17-0900-82; RRID: AB_1272252 |
| CD90.1-Pacific Blue |
eBioscience |
Clone: HIS51; Cat# 48-0900-80; RRID: AB_1272254 |
| EOMES-PE |
eBioscience |
Clone: Dan11mag; Cat# 12-4875-80; RRID: AB_1603275 |
| CD4-PE/Cy7 |
Biolegend |
Clone: GK5.1; Cat# 100422; RRID: AB_312707 |
| CD4-APC |
Biolegend |
Clone: GK5.1; Cat# 100412; RRID: AB_312697 |
| CD4-APC/Cy7 |
Biolegend |
Clone: GK5.1; Cat# 100414; RRID: AB_312699 |
| CD8-PE/Cy7 |
Biolegend |
Clone: 53-6.7; Cat# 100722; RRID: AB_312761 |
| CD8-APC |
Biolegend |
Clone: 53-6.7; Cat# 100712; RRID: AB_312751 |
| CD8-FITC |
Biolegend |
Clone: 53-6.7; Cat# 100706; RRID: AB_312745 |
| IL17-PE |
Biolegend |
Clone: TC11-18H10.1; Cat# 506904; RRID: AB_315464 |
| IL17-Pacific Blue |
Biolegend |
Clone: TC11-18H10.1; Cat# 506918; RRID: AB_893544 |
| IFNγ-PE |
Biolegend |
Clone: XMG1.2; Cat# 505808; RRID: AB_315402 |
| IFNγ-Alexa647 |
Biolegend |
Clone: XMG1.2; Cat# 505814; RRID: AB_493314 |
| T-bet-PE |
Biolegend |
Clone: 4B10; Cat# 644809; RRID: AB_2028583 |
| CCR5-PE |
Biolegend |
Clone: HM-CCR5; Cat# 107005; RRID: AB_313300 |
| CCR6-PE |
Biolegend |
Clone: 29-2L17; Cat# 129803; RRID: AB_1279139 |
| CCR7-PE |
Biolegend |
Clone: 4B12; Cat# 120105; RRID: AB_389357 |
| CD73-Pacific Blue |
Biolegend |
Clone: Ty/11.8; Cat# 127212; RRID: AB_11219190 |
| CD38-PE |
Biolegend |
Clone: 90; Cat# 102707; RRID: AB_312928 |
| CD38-APC |
Biolegend |
Clone: 90; Cat# 102711; RRID: AB_312932 |
| CD71-PE/Cy7 |
Biolegend |
Clone: RI7217; Cat# 113811; RRID: AB_2203383 |
| PD1-PE |
Biolegend |
Clone: RMP1-30; Cat# 109103; RRID: AB_313420 |
| PD1-PE/Cy7 |
Biolegend |
Clone: RMP1-30; Cat# 109109; RRID: AB_572016 |
| CD98-PE |
Biolegend |
Clone: RL388; Cat# 128207; RRID: AB_1186107 |
| Annexin V FITC |
BD Biosciences |
Clone: N/A; Cat# 51-65874X; RRID: N/A |
| CD45.1-APC |
BD Biosciences |
Clone: A20; Cat# 558701; RRID: AB_1645214 |
| Vβ13-FITC |
BD Biosciences |
Clone: MR1 2-3; Cat# 553204; RRID: AB_394706 |
| Vβ14-FITC |
BD Biosciences |
Clone: 14-2; Cat# 553258; RRID: AB_394738 |
| Vβ12-FITC |
Thermo Fisher Scientific |
Clone: S511; Cat# TCR2654; RRID: AB_417092 |
| Slc1a5 |
Thermo Fisher Scientific |
Clone: N/A; Cat# PA5-50527; RRID: AB_2635980 |
| Anti-Goat Alexa647 |
Thermo Fisher Scientific |
Clone: N/A; Cat# A21447; RRID: AB_141844 |
| pFoxo1 (S256) |
Cell Signaling Technology |
Clone: N/A; Cat# 9461; RRID: AB_329831 |
| Phospho-S6 Ribosomal Protein (Ser235/236)-Alexa647 |
Cell Signaling Technology |
Clone: D57.2.2E; Cat# 4851; RRID: AB_916160 |
| Hexokinase I (HK I) |
Cell Signaling Technology |
Clone: C35C4; Cat# 2024S; RRID: AB_2116996 |
| Hexokinase II (HK II) |
Cell Signaling Technology |
Clone: C64G5; Cat# 2867S; RRID: AB_2232946 |
| Platelet-type phosphofructokinase (PFKP) |
Cell Signaling Technology |
Clone: D4B2; Cat# 8164S; RRID: N/A |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
Cell Signaling Technology |
Clone: D16H11; Cat# 5174S; RRID: AB_10622025 |
| Lactate dehydrogenase (LDH) |
Cell Signaling Technology |
Clone: C4B5; Cat# 3582S; RRID: AB_2066887 |
| Pyruvate kinase M 1/2 (PKM1/2) |
Cell Signaling Technology |
Clone: C103A3; Cat# 3190S; RRID: AB_2163695 |
| Pyruvate Dehydrogenase |
Cell Signaling Technology |
Clone: C54G1; Cat# 3205S; RRID: AB_2162926 |
| Glutamate Dehydrogenase 1/2 (Glud1/12) |
Cell Signaling Technology |
Clone: D9F7P; Cat# 12793S; RRID: N/A |
| Foxo1 |
Cell Signaling Technology |
Clone: C29H4; Cat# 2880S; RRID: AB_2106495 |
| Acetylated-Lysine |
Cell Signaling Technology |
Clone: N/A; Cat# 9441S; RRID: N/A |
| Anti-Rabbit HRP |
Cell Signaling Technology |
Clone: N/A; Cat# 7074S; RRID: N/A |
| PGC1 α-Alexa647 |
Novus Biologicals |
Clone: N/A; Cat# NBP1-04676AF647; RRID: N/A |
| Glutaminase (Gls) |
Novus Biologicals |
Clone: N/A; Cat# NBP2-29940; RRID: N/A |
| Anti-Rabbit PE |
Jackson ImmunoResearch Laboratories |
Clone: N/A; Cat# 111-116-144; RRID: AB_2337985 |
| Anti-Rabbit Alexa647 |
Jackson ImmunoResearch Laboratories |
Clone: N/A; Cat# 111-607-003; RRID: AB_2338084 |
| xCT |
Santa Cruz Biotechnology |
Clone: Q-18; Cat# Sc79360; RRID: AB_2190856 |
| InVivoMab anti-mouse IL-17A |
BioXCell |
Clone: 17F3; Cat# BE0173; RRID: AB_10950102 |
| InVivoMAb anti-mouse IFNγ |
BioXCell |
Clone: XMG1.2; Cat# BE0055; RRID: AB_1107694 |
| Anti CD38 VHH Single Domain Antibody |
Creative Biolabs |
Clone: N/A; Cat# NABL-079; RRID: N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| FK866 |
Cayman Chemical |
Cat# 13287 |
| Ex527 |
Cayman Chemical |
Cat# 10009798 |
| Etomoxir |
Cayman Chemical |
Cat# 11969 |
| AKTi |
Cayman Chemical |
Cat# 14870 |
| SC-79 |
Sigma Aldrich |
Cat# SML0749 |
| 6-Diazo-5-oxo-L-norleucine (DON) |
Sigma Aldrich |
Cat# D2141 |
| 2-Deoxy-D-glucose (2DG) |
Sigma Aldrich |
Cat# D6134 |
| Antimycin A |
Sigma Aldrich |
Cat# A8674 |
| Rotenone |
Sigma Aldrich |
Cat# R8875 |
| Oligomycin |
Sigma Aldrich |
Cat# O4876 |
| FCCP |
Sigma Aldrich |
Cat# C2920 |
| IMDM |
GE Healthcare, HyClone |
Cat# SH30228.01 |
| RPMI-1640 (Glucose free) |
Thermo Fisher Scientific |
Cat# 11879-020 |
| RPMI-1640 (glutamine free) |
Thermo Fisher Scientific |
Cat# 21870-076 |
| Penicillin-Streptomycin |
Corning |
Cat# 30-001-CI |
| Fetal Bovine Serum (FBS) |
Atlanta Biologicals |
Cat# S11150
|
| rIL12 |
Biolegend |
Cat# 577004 |
| rIL6 |
Biolegend |
Cat# 575704 |
| rIL1β |
Biolegend |
Cat# 575104 |
| rIL23 |
Biolegend |
Cat# 589002 |
| rTGFβ |
Biolegend |
Cat# 580702 |
| rhIL2 |
NCI, Biological Resources Branch |
https://ncifrederick.cancer.gov/research/brb/productDataSheets/cytokineHumanInterleukins/IL-2Bulk.aspx |
| Foxp3 / Transcription Factor Staining Buffer Set |
Thermo Fisher Scientific |
Cat# 00-5523 |
| Fixation/Permeabilization Solution Kit |
BD Biosciences |
Cat# 554714 |
| Mouse CD4+ T cell isolation kit |
Miltenyi Biotec |
Cat# 130-104-454 |
| hgp10025–33 peptide (KVPRNQDW) |
Genscript |
Cat# RP20344 |
| TRP-1106–130 peptide (SGHNCGTCR PGWRGAACNQKILTVR) |
NeoMPS |
Custom synthesized |
| TCR-I peptide (SAINNYAQKL) |
Sydlabs |
Cat# 075472 |
| RIPA Lysis Buffer |
Thermo Fisher Scientific |
Cat# 89900 |
| NE-PER Nuclear and Cytoplasmic Extraction Reagents |
Thermo Fisher Scientific |
Cat# 78833 |
| Critical Commercial Assays |
| CellTrace CFSE Cell Proliferation Kit |
Thermo Fisher Scientific |
Cat# C34554
|
| CellTrace Violet Cell Proliferation Kit |
Thermo Fisher Scientific |
Cat# C34557
|
| NAD/NADH Cell-Based Assay Kit |
Cayman Chemical |
Cat# 600480 |
| Universal SIRT Activity Assay Kit |
abcam |
Cat# ab156915 |
| TransAM FKHR (FOXO1) Transcription Factor ELISA Kits |
Active Motif |
Cat# 46396 |
| Akt kinase activity kit |
Enzo Life Sciences |
Cat# ADI-EKS-400A |
| Immunoprecipitation (IP) Kit |
Biovision |
Cat# K286-25 |
| iScript cDNA Synthesis Kit |
Biorad |
Cat# 1708891 |
| SsoAdvanced Universal SYBR Green Supermix |
Biorad |
Cat# 1725274 |
| Deposited Data |
| Gene microarray dataset |
This paper |
ArrayExpress accession # E-MTAB-5237 |
| Experimental Models: Cell Lines |
| B16-F10 |
ATCC |
CRL-6475 |
| B16-F10-A2+
|
Rolf Kiessling, Karolinska Institute, Stockholm, Sweden. Mycoplasma testing was done in Mehrotra lab. |
N/A |
| Experimental Models: Organisms/Strains |
| C57BL/6 |
Jackson Laboratory |
Stock# 000664 |
| C57BL/6-Tg(HLA-A2.1)1Enge/J |
Jackson Laboratory |
Stock# 003475 |
| B6.129S7-Rag1tm1Mom/J |
Jackson Laboratory |
Stock# 002216 |
| B6.129P2-Cd38tm1Lnd/J |
Jackson Laboratory |
Stock# 003727 |
| B6;129-Sirt1tm1Ygu/J |
Jackson Laboratory |
Stock# 008041 |
| Tg(Cd4-cre)1Cwi/BfluJ |
Jackson Laboratory |
Stock# 017336 |
| B6.SJL-Ptprca Pepcb/BoyJ |
Jackson Laboratory |
Stock# 002014 |
| B6.129S7-Ifngtm1Ts/J |
Jackson Laboratory |
Stock# 002287 |
| B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J |
Jackson Laboratory |
Stock# 008684 |
| B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J |
Jackson Laboratory |
Stock# 005023 |
| C57BL/6-Tg(TRAMP)8247Ng/J |
Jackson Laboratory |
Stock# 003135 |
| IFNγThy1.1 knockin |
Casey T. Weaver, University of Alabama at Birmingham (UAB) |
(Harrington et al., 2008) |
| Foxo1fl/flLckcre
|
Melanie Gubbels Bupp, Randolph-Macon College, VA |
(Gubbels Bupp et al., 2009)
|
| TCR-I |
Jennifer Wu, MUSC |
(Staveley-O’Carroll et al., 2003)
|
| Oligonucleotides |
| Primers for qPCR, see Table S4
|
| Recombinant DNA |
| MSGV1-TRP-1 vector |
(Kerkar et al., 2011) |
N/A |
| LV-TCR/sr39TK-GFP vector |
(Koya et al., 2010) |
N/A |
| Software and Algorithms |
| FlowJo 10.2 |
TreeStar, OR |
https://www.flowjo.com/solutions/flowjo/downloads/ |
| Prism 5 |
GraphPad |
https://www.graphpad.com/scientiflc-software/prism/ |
| Agilent Seahorse Wave 2.4 |
Agilent |
http://www.agilent.com/en-us/products/cell-analysis-(seahorse)/seahorse-wave-software |
| CFX Manager 3.1 |
Biorad |
http://www.bio-rad.com/en-us/sku/soft-cfx-31-patch-cfx-manager-software-v3-1-upgrade |
| GenomeStudio software |
Illumina |
https://www.illumina.com/techniques/microarrays/array-data-analysis-experimental-design/genomestudio.html |
| PIMENTo |
(Sásik et al., 2004) |
https://github.com/MUSC-CGM/PIMENTo |
| ToppGene Suite |
(Chen et al., 2009) |
https://toppgene.cchmc.org/ |
| Venny |
This paper |
http://bioinfogp.cnb.csic.es/tools/venny/index.html |
