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A mixed lymphocyte-endothelial reaction was performed in a standard 96-well plate. MNC were plated alone, with allogeneic vascular endothelial cells (VEC) or on Immunocloaked (IC) VEC. Cells were allowed to proliferate for 4 days prior to pulsing with [3H] thymidine. The cells were then allowed to incubate for 18 hours and read on a scintillation counter (cpm).
