Table 3. Primer sequence.
Primer | Sequence (5′-3′) |
---|---|
PGK Crisp L1 | TCTCGTGCAGATGGACAGCA |
Neo Crisp R1 | CGGCAGGAGCAAGGTGAGAT |
Bsd Crisp R1 | GGGCAGCAATTCACGAATCC |
Bxb1 attR 2L | GGCCGTGATGACCTGTGTCTT |
MICRISP Neo 2R | TGTGCCCAGTCATAGCCGAAT |
R4 att R 2L | TGTTCCCCAAAGCGATACCAC |
MICRISPR Bsd 2R | GCTGGCGACGCTGTAGTCTTC |
3’HPRT L1 | GTGAATCTTTGTCAGCAGTTCCCTTTTA |
MI Hyg R1 | GCAAAGTGCCGATAAACATAACGATCT |
PGK Crisp L1 | TCTCGTGCAGATGGACAGCA |
Neo Crisp R1 | CGGCAGGAGCAAGGTGAGAT |
Bsd Crisp R1 | GGGCAGCAATTCACGAATCC |
Hyg Crisp L1 | GCAGCTTTGCTCCTTCGCTTT |
Hyg Crisp R1 | CAGGCAGGTCTTGCAACGTG |