Table 2. Evaluated immunoassay kits, including assay characteristics and CSF dilutions.
| Commercial panel name | Company | Catalog number | Biomarker targets | CSF Dilution | |
|---|---|---|---|---|---|
| Core AD assays | |||||
| V-PLEX Plus Human Total Tau | MSD | K151LAG | total tau | 1:4 | |
| V-PLEX Plus Aβ Peptide Panel 1 (6E10) | MSD | K15200G | Aβ1–38, Aβ1–40, Aβ1–42 | 1:2 | |
| Aβ and tau-independent neurodegeneration assays | |||||
| U-PLEX Plus Human α-Synuclein | MSD | K151WKP | α-Synuclein | 1:8 | |
| Human FABP3 | MSD | K151HTD | FABP3 | Neat† | |
| NfL (Neurofilament-light) ELISA | UmanDiagnostics | 10–7002 | Neurofilament-light | 1:2 | |
| Metabolic assays | |||||
| 24(S)-Hydroxycholesterol ELISA | Enzo Life Sciences | ADI-900-210-0001 | 24-OHC | 1:2 | |
| Human Active GLP-1 (7–36) amide, Insulin, Glucagon, Leptin | MSD | K15173C | Active GLP-1 (7–36) amide, Insulin, Glucagon, Leptin | Neat† | |
| Human Adiponectin | MSD | K151BXC | Adiponectin | 1:10 | |
| Insulin Receptor Human ELISA | BioVendor | RD1991041200R | Soluble Insulin Receptor | 1:2† | |
| VGF Nerve Growth Factor Inducible (VGF) Human ELISA | Hölzel Diagnostika | SEB166Hu | VGF | Neat† | |
| Oxidative stress assays | |||||
| OxiSelect CML Competitive ELISA | Cell Biolabs, Inc. | STA-816 | CML | Neat† | |
| OxiSelect Oxidative DNA Damage ELISA | Cell Biolabs, Inc. | STA-320 | 8-OHdG | Neat† | |
| Neuroinflammatory and vascular injury assays | |||||
| V-PLEX Plus Angiogenesis panel 1 (human) | MSD | K15190G | FGF (basic), Flt-1, PlGF, Tie-2, VEGF-C, VEGF-D | Neat† | |
| V-PLEX Plus Chemokine Panel 1 (human) | MSD | K15047G | Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Neat† | |
| V-PLEX Plus Cytokine panel 1 (human) | MSD | K15050G | IL-1α, IL-5, IL-7, IL-12/IL-23p40, IL-15, IL-16, IL-17A, TNF-β, VEGF | Neat† | |
| Human MMP 2-Plex Ultra-Sensitive Panel | MSD | K15033C | MMP-2, MMP-10 | Neat† | |
| Human MMP 3-Plex Ultra-Sensitive Panel | MSD | K15034C | MMP-1, MMP-3, MMP-9 | Neat† | |
| V-PLEX Plus Proinflammatory Panel 1 (human) | MSD | K15049G | IFN-ɣ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-13, TNF-α | Neat† | |
| V-PLEX Plus Vascular Injury Panel 2 (human) | MSD | K15198G | CRP, ICAM-1, SAA, VCAM-1 | 1:5 | |
| Human YKL-40 | MSD | K151NHD | YKL-40 | 1:50† | |
† Datasheet only included published dilution factors for serum and plasma. Dilution factor required for CSF was determined experimentally. Abbreviations: MSD, Meso Scale Diagnostics, LLC.