Fig 2. Nitrite increased P-VASP^Ser239 but not P-VASP^Ser157 in the presence of deoxygenated erythrocytes.

Quantification of baseline P-VASP^Ser239/ VASP (A) and P-VASP^Ser157/ VASP (B) in platelets from PRP, PRP + erythrocytes (20% hematocrit) with or without deoxygenation, representative Western blot bands of P-VASP^Ser239 (C), P-VASP^Ser157 (D), VASP expression and quantification of P-VASP^Ser239/VASP (E) and P-VASP^Ser157/VASP (F) in control and nitrite treated deoxygenated PRP + erythrocytes (20% hematocrit) at different time points are shown in this figure. PRP + erythrocytes (20% hematocrit) were deoxygenated by helium for 10 minutes (pO₂ ~ 25 mmHg). Nitrite (10 μM) was added in deoxygenated samples and incubated for 5, 10, 15, 20, 30, 40 and 60 minutes. Data are mean ± SEM (n = 7). *P < 0.05 compared with PRP and tested by one-way ANOVA with Tukey’s multiple comparison. ^#P < 0.05 compared with control and tested by paired Student’s t-test.