Fig 4. Erythrocytes diminished P-VASP^Ser239 induced by NO donors.

Representative Western blot bands of P-VASP^Ser239 and VASP expression in platelets (A) and quantification of P-VASP^Ser239/VASP (B) and fold increase of P-VASP^Ser239 expression (C) in platelets in PRP and whole blood treated with DEANONOate and sodium nitroprusside are shown. DEANONOate (1 μM) and sodium nitroprusside (10 μM) were incubated in PRP or venous whole blood (WB) at 37 °C for 5 minutes. Data are mean ± SEM (n = 4). *P < 0.05 compared with baseline (BL) and tested by one-way ANOVA with Tukey’s multiple comparison. ^#P < 0.05 compared with PRP and tested by paired Student’s t-test. DEA/NO = DEANONOate, SNP = Sodium nitroprusside.