Fig 5. Comparative effects of nitrite and NO donors in deoxygenated whole blood.
Quantification of P-VASPSer239/VASP at baseline (A) and after treatment of deoxygenated whole blood with nitrite, DEANONOate and sodium nitroprusside (B) are shown. Whole blood was deoxygenated by helium for 10 minutes. Nitrite (10 μM) was incubated in deoxygenated whole blood for 15 minutes while DEANONOate (1 μM) and sodium nitroprusside (10 μM) were incubated for 5 minutes, respectively. Data are mean ± SEM (n = 5). *P < 0.05 compared with PRP and tested by one-way ANOVA with Tukey’s multiple comparison. #P < 0.05 compared with control and tested by one-way ANOVA with Tukey’s multiple comparison.