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. 2018 Feb 16;7:16. doi: 10.1038/s41426-018-0034-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Serum HBsAb titers increased persistently in all immunized healthy subjects at day 7 and day 28 post HBV vaccine boost. b Representative B-cell ELISpot readouts (HC18), who had already finished HBV vaccine immunization 2 years ago, at day 0 (top), day 7 (middle) and day 28 (bottom) after HBV vaccine boost were shown. c HBs-specific B-cell responses were measured either at day 7 or day 28 after boost. *P < 0.05