Fig. 1. Co-expression of P1 and 3CD of EV-D68 in P. pastoris.
a Schematic diagrams of the constructs made in this study. TRP2-L and TRP2-R, the up-stream and down-stream parts of the TRP region, used for homologous recombination; PAOX1, AOX1 promoter; CYC1 TT, CYC1 transcription termination region; ADE2, expression cassette encoding phosphoribosylaminoimidazole carboxylase, used as the selection marker. b ELISA analysis of cell lysates from pEV-D68-003-transformed yeast clones. A polyclonal antibody against inactivated EV-D68 was used for detection. Cell lysate from a yeast clone transformed with the empty vector pPink-HC was used as the negative control (ctr). Data are mean ± SD of triplicate wells. c Western blotting analysis with an anti-VP3 polyclonal antibody. Lane M, marker; lane 1, empty vector-transformed yeast; lane 2, pEV-D68-003-transformed yeast; lane 3, inactivated EV-D68 virus