Figure 2.

Analysis of inflammatory mediators in striatal extracts obtained from wild type and Nlrp3^−/− mice exposed to rotenone. A and B, Freshly dissected striatal tissues were obtained from wild type and Nlrp3^−/− mice exposed to either vehicle and rotenone following 6 months of exposure and homogenized for inflammatory cytokine analysis. Extracts were analyzed in triplicate using a 32-plex multiple cytokine bead based assay. Z-scored data were analyzed for global changes, significant overall treatment effects were observed when comparing the inflammatory cytokine signature of rotenone treated wild type animals to vehicle treated groups at each time point (p value < .001, 2-way ANOVA, n = 4 per group). Asterisks indicate individual cytokines identified using this screen as having significantly altered levels as compared with vehicle controls (Dunnet’s Multiple Comparison Test, p value* < .05). C and D, Representative immunoblots generated following SDS-PAGE analysis of striatal extracts obtained from treatment groups described above indicating elevated Nlrp3 expression and caspase 1 cleavage in wild type mice exposed to rotenone (n = 4 per group).