Skip to main content
. Author manuscript; available in PMC: 2018 Mar 5.
Published in final edited form as: Birth Defects Res B Dev Reprod Toxicol. 2011 Nov 29;95(1):79–88. doi: 10.1002/bdrb.20344

Fig. 4.

Fig. 4

Prompt alterations of expression for Shh and Bmp4 induced by RA administration. A–E: Rarb expression level is upregulated 2 hr after RA administration (A and B). Such upregulated expression continued for 4 hr (C), and decreased 6 hr (D and E) after RA treatment. F–I: Shh expression level is increased 2 hr (F and G) after RA treatment, sustained at high levels for 6 hr (H and I), and slightly decreased 8 hr after RA treatment (J). K–O: Ptc1 expression is unaltered within 4 hr of RA treatment (K–M), then prominently increased 6 and 8 hr after RA treatment (N and O). P–T: Bmp4 expression is upregulated 2 hr after RA treatment, and sustained with increased level until 8 hr after RA treatment. U and V: Cyp26b1 expression is upregulated in the GT mesenchyme and ectoderm epithelium 6 hr after RA administration. W and X: Meis2 expression in the ventral bilateral mesenchyme of the UE (indicated by black arrows) is reduced 6 hr after RA treatment. All images are placed ventral side down. Scale bar, 400 μm. GT, genital tubercle; RA, retinoic acid; UE, urethral plate epithelium.