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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Hypertens. 2017 Sep;35(9):1872–1880. doi: 10.1097/HJH.0000000000001373

Figure 2. Expression of miRNAs potentially targeting PHD2 mRNA in the renal medulla and interaction of miR-429 with 3′-UTR segment of PHD2 in cultured RMIC cells.

Figure 2

A: High salt intake increased the level of miR-429. * P<0.05 vs. LS. LS, low salt; HS, high salt. (n=5). B: miR-429 decreased luciferase activity when the luciferase gene was constructed with the 3′-UTR segment of PHD2. RMIC cells were co-transfected with rat PHD 2 3′-UTR luciferase reporter constructs RmiT or control 3′-UTR CmiT, and miR-429 precursor (miR-429) or scrambled miRNA precursor (Ctrl). * P<0.05 vs. cells treated with CmiT. ** P<0.05 vs. cells treated with RmiT and Ctrl. RmiT, construct of luciferase reporter with 3′-UTR of rat PHD2; CmiT, construct of luciferase reporter with scrambled 3′-UTR; Ctrl, scrambled miRNA precursor; miR-429, miRNA-429 precursor (n=4–6).