Skip to main content
. 2017 Jan 29;140(3):582–598. doi: 10.1093/brain/aww357

Figure 2.

Figure 2

FXS FMR1− lines exhibit aberrant neurogenic phenotypes. (A) Representative images of non-disease, FXS FMR1+ and FXS FMR1− cultures at Day 12 of differentiation. FXS FMR1− hiPSCs generate fewer βIII tubulin+ (Tuj1) immature neurons that have longer primary neurites relative to non-disease and FXS FMR1+ lines. Scale bar = 100 µm. (B) Quantitation of the number of βIII tubulin+ cells produced at D12 by combined FMR1+ hPSC, and FXS FMR1− hiPSCs. Normalized to total number of cells. P = 2.0 × 10−8. (C) Quantitation of neurite length at D12 by combined FMR1+ hPSC, and FXS FMR1− hiPSCs. Normalized to number of βIII tubulin+ cells. P = 0.02. For quantitation in B and C, n = 3 region of interest each from biological replicates of four FMR1+ hPSC lines (one non-disease, three FXS FMR1+ lines) compared to four FXS FMR1− lines. Error bars indicate SEM.