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. 2017 May 15;158(2):334–346. doi: 10.1093/toxsci/kfx094

Figure 4.

Figure 4

TCA increases mRNA levels of pro-inflammatory modulators in primary hepatocytes isolated from Fxr−/− mice. A, Primary hepatocytes isolated from Fxr−/− and WT mice were treated with DCA (100 μM), TCA (100 μM), T-β-MCA (100 μM), or the same volume of vehicle (dimethyl sulfoxide) for 6 h. Levels of Ccl2, Pai1, and Tnf mRNAs were measured by qPCR. The mRNA levels were expressed as the relative values to those of vehicle-treated WT hepatocytes. B, Primary hepatocytes isolated from Fxr−/− and WT mice were treated with different concentrations of TCA (10, 30, and 100 µM) or vehicle for 6 h. The mRNA levels were expressed as the relative values to those of vehicle-treated WT hepatocytes. *P<.05; **P<.01; ***P<.001.