Effect of CLOF on mRNA of BA regulators in livers and ilea of WT and PPARα-null mice. CO or CLOF (300 mg/kg) was administered daily (i.p.) for 4 consecutive days to male WT and PPARα-null mice (n = 6–8 per treatment group). QuantiGene Plex 2.0 Assay was performed to measure the gene expression of Fxr, Shp, Lrh-1 and Fgfr4 in the liver and Fxr, Shp, Fgf15, and Tgr5 in the ileum. Relative mRNA levels were calculated with vehicle-treated WT male mice set as 100%. Data represent means ± SEM *p < .05 versus vehicle treatment group.