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. 2017 May 8;158(2):263–274. doi: 10.1093/toxsci/kfx095

Figure 2.

Figure 2

Pulmonary acrolein stress and induction of acrolein-metabolizing enzymes. Mice were exposed to HEPA-filtered air (n = 8) or acrolein (1 ppm, 6 h/day, 5 days/week; n = 8) for 12 weeks. Lung proteins were analyzed by Western blotting as described under Materials and Methods. A, Protein-acrolein adducts probed with anti-KLH-acrolein antibody (n = 8/group). B, Abundance of acrolein-metabolizing enzymes: GSTP, AR, and ALDH2 in air- and acrolein-exposed mice (n = 8/group). Values are mean ± SEM. *p < .05 vs. Air.