Figure 5.
Abundance of circulating blood immune cells of acrolein-exposed mice. Mice were exposed to HEPA-filtered air (open bars) or acrolein (0.5 [gray bars] or 1ppm [black bars], 6 h/day, 5 days/week) for 12 weeks. A, Flow cytometry gating scheme for immune cell differentials. Levels of: B, GR1+ granulocytes; C, NK1.1+ natural killer cells; D, CD19+ B-cell lymphocytes; E, CD4+ and CD8+ T-cell lymphocytes; F, CD11b+ monocytes; and G, Ly6Chigh and Ly6Clow subsets of monocytes in blood of mice exposed to air or acrolein for 12 weeks (n = 14–15/group). Values are mean ± SEM. *p < .05 vs. Air.