Table 2.
Biochemical tests | Analysis method/platform used |
---|---|
Fasting glucose | Analysed using Instrumentation Laboratory IL TestTM kits on an ILab 600/650 clinical chemistry analysers (Werfen, Warrington, UK) |
Total cholesterol | |
Triglycerides | |
HDL- and LDL-cholesterol | Analysed using Randox kits adapted for use on the Ilab 600/650 analysers (Randox Laboratories, Crumlin, Northern Ireland) |
Non-esterified fatty acids (NEFA) | |
Apolipoprotein-B | |
Apolipoprotein A1 | |
Glycerol | |
Lactate | |
3-hydroxybuthyrate | |
C-reactive protein | Analysed using a Siemens ADVIA wide range CRP kit adapted for use on the Ilab 600/650 analysers. (Siemens Healthcare Diagnostics, Camberley, UK) |
Fasting insulin | Millipore Human Insulin specific radioimmunoassay (Millipore UK, Watford, UK) |
Adiponectin | Perkin Elmer AlphaLisa Human Adiponectin kit (Waltham, MA, USA) |