FIG. 8.

Cholix-induced PARP cleavage is not enhanced by TNF-α in immortalized human hepatocytes. A–D, Immortalized human hepatocytes were pretreated with or without the inhibitors for 30 min, and then incubated for 12 h with wild-type Cholix (WT) or mutant Cholix(E581A) (MT) in the presence or absence of TNF-α. Then, cells were lysed with 1×SDS sample buffer for immunoblotting with the indicated antibodies. A blot representative of three separate experiments is shown.