Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
strain, strain background (S. cerevisiae)	Yeast Strains	in this study		See Supplementary file 1 Yeast strains
strain, strain background (E.coli)	E. coli	Thermo Fisher	Cat#18265017	E. coli DH5α Competent Cells
antibody	H3	Abcam	Ab1791; RRID: AB_302613	Anti-Histone H3
antibody	H3K36me3	Abcam	Ab9050; RRID: AB_306966	Anti-Histone H3 tri methyl K36
commercial assay or kit	QIAprep Spin Miniprep Kit	QIAGEN	Cat#27106
commercial assay or kit	Wizard SV Gel and PCR Clean-Up System	Promega	Cat#A9182
commercial assay or kit	MasterPure Yeast RNA Purification Kit	Lucigen	MPY03100
chemical compound, drug	Protein A magnetic beads	GenScript	Cat. No. L00273
chemical compound, drug	Phusion high-fidelity PCR master mix	NEB	cat. no. M0531S
chemical compound, drug	GoTaq qPCR Master Mix	Promega	Cat#A6002
chemical compound, drug	NAT	Jena Bioscience	AB-102L	Nourseothricin dihydrogen, cloNAT
chemical compound, drug	G418	Duchefa-biochemie	G0175	G-418 disulphate
chemical compound, drug	5FOA	Thermo Fisher	R0811	5-Fluoroorotic Acid
software, algorithm	ImageQuant	GE Healthcare	NA
software, algorithm	Software for flow cytometry and bioinformatic analysis: see Materials and methods	NA	NA
other	Oligonucleotides	in this study	NA	See Supplementary file 2 Oligonucleotides
other	Equipment: BD Fortessa	BD biosciences	NA
other	Plasmids	in this study	NA	See Supplementary file 3 Plasmids