Cholesterol depletion by MβCD sensitizes P3 neonates to BoNT/A. A, Samples traces of stimulus evoked EPP for control, MβCD (10 mM for 30 min), BoNT/A (10 pM) and MβCD (pretreatment for 30 min) followed by BoNT/A (10 pM; 90 min) treated conditions. All measurements were made at the end of 90 min of each treatment. B and C, Bar graphs represent the summary of mean EPP and mean mEPP ± SD in these NMPs at the end of 90 min of treatment (n = 8 neonates, 8 NMPs, and 22 endplates).